Half Title Page.
Methods in Enzymology.
1: Expression of GPCRs in Pichia Pastoris for Structural Studies.
2: Conformational Ensemble View of G Protein-Coupled Receptors and the Effect of Mutations and Ligand Binding.
3: Structural Evolution of G-Protein-Coupled Receptors.
4: Directed Evolution of G-Protein-Coupled Receptors for High Functional Expression and Detergent Stability.
5: The Role of Hydrophobic Amino Acids in the Structure and Function of the Rhodopsin Family of G Protein-Coupled Receptors.
6: Structure of β-Adrenergic Receptors.
7: Advances in Methods to Characterize Ligand-Induced Ionic Lock and Rotamer Toggle Molecular Switch in G Protein-Coupled Receptors.
8: Crystallogenesis of Adenosine A2A Receptor—T4 Lysozyme Fusion Protein.
9: Probing GPCR Structure.
10: Strategies for Studying the Ligand Binding Site of GPCRs: Photoaffinity Labeling of the VPAC1 Receptor, a Prototype of Class B GPCRs.
11: Expression of Mammalian G Protein-Coupled Receptors in Caenorhabditis elegans.
12: Expression, Purification, and Structural Analysis of Intracellular C-Termini from Metabotropic Glutamate Receptors.
13: Unnatural Amino Acid Mutagenesis of GPCRs Using Amber Codon Suppression and Bioorthogonal Labeling.
14: Mapping a Ligand Binding Site Using Genetically Encoded Photoactivatable Crosslinkers.
15: Alternative mRNA Splicing of G Protein-Coupled Receptors.
16: Functional Residues Essential for the Activation of the CB1 Cannabinoid Receptor.