Cilia, Part B, 1st Edition

  • Volume 2
  • Published By:
  • ISBN-10: 0123984858
  • ISBN-13: 9780123984852
  • DDC: 571.67
  • Grade Level Range: College Freshman - College Senior
  • 498 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013

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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
Methods in Enzymology.
1: Non-Motile Primary Cilia as Fluid Shear Stress Mechanosensors.
2: Biomechanical Measurement of Kinocilium.
3: Analysis of Primary Cilia in Directional Cell Migration in Fibroblasts.
4: Sperm Patch-Clamp.
5: Laser Trap Measurements of Flagellar Membrane Motility.
6: Probing Ciliogenesis Using Micropatterned Substrates.
7: Fluorescent Imaging of Drosophila melanogaster Sperm in the Reproductive Tract: A New Model of Flagellar Motility.
8: Visualization of Mouse Nodal Cilia and Nodal Flow.
9: Dissecting the Functional Interplay between the TOR Pathway and the Cilium in Zebrafish.
10: Using Xenopus Skin to Study Cilia Development and Function.
11: Zebrafish Cilia.
12: Analysis of Ciliary Assembly and Function in Planaria.
13: Discovery and Functional Evaluation of Ciliary Proteins in Tetrahymena Thermophila.
14: Analysis of Ciliogenesis in Primary Culture Mouse Tracheal Epithelial Cells.
15: Isolation of Mammalian Primary Cilia.
16: Finding Ciliary Genes:A Computational Approach.
17: Chemical Screening Methods for Flagellar Phenotypes in Chlamydomonas.
18: Centrosome Isolation and Analysis by Mass Spectrometry-Based Proteomics.
Color Plate.
Author Index.
Subject Index.