Microtubules, in vitro, 2nd Edition

  • Published By:
  • ISBN-10: 0124078885
  • ISBN-13: 9780124078888
  • DDC: 571.654
  • Grade Level Range: College Freshman - College Senior
  • 440 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013

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There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This 2e brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors.

Table of Contents

Front Cover.
Half Title Page.
Series Page.
Title Page.
Copyright Page.
1: Site-Specific Fluorescent Labeling of Tubulin.
2: Measuring Microtubule Persistence Length Using a Microtubule Gliding Assay.
3: Structural Studies of the Doublecortin Family of Maps.
4: Detection and Quantification of Microtubule Detachment from Centrosomes and Spindle Poles.
5: Regulation of Tubulin Expression by Micro-RNAs: Implications for Drug Resistance.
6: Determining the Structure–Mechanics Relationships of Dense Microtubule Networks with Confocal Microscopy and Magnetic Tweezers-Based Microrheology.
7: Studying Mitochondria and Microtubule Localization and Dynamics in Standardized Cell Shapes.
8: Going Solo: Measuring the Motions of Microtubules with an In Vitro Assay for TIRF Microscopy.
9: Analysis of Microtubules in Isolated Axoplasm from the Squid Giant Axon.
10: Imaging GTP-Bound Tubulin: From Cellular to In Vitro Assembled Microtubules.
11: Tubulin-Specific Chaperones: Components of a Molecular Machine That Assembles the α/β Heterodimer.
12: Heterotrimeric G Proteins and Microtubules.
13: Purification and Biophysical Analysis of Microtubule-Severing Enzymes In Vitro.
14: Measurement of In Vitro Microtubule Polymerization by Turbidity and Fluorescence.
15: Live-Cell Imaging of Microtubules and Microtubule-Associated Proteins in Arabidopsis Thaliana.
16: Investigating Tubulin Posttranslational Modifications with Specific Antibodies.
17: Purification and Assembly of Bacterial Tubulin Btuba/b and Constructs Bearing Eukaryotic Tubulin Sequences.
18: Microtubule-Associated Proteins and Tubulin Interaction by Isothermal Titration Calorimetry.
19: Methods for Studying Microtubule Binding Site Interactions: Zampanolide as a Covalent Binding Agent.
20: Studying Kinetochore-Fiber Ultrastructure Using Correlative Light-Electron Microscopy.
21: Fluorescence-Based Assays for Microtubule Architecture.
22: Structure–Function Analysis of Yeast Tubulin.
23: Using Mtbindingsim as a Tool for Experimental Planning and Interpretation.
24: Imaging Individual Spindle Microtubule Dynamics in Fission Yeast.
Volumes in Series.