The Immunoassay Handbook, 4th Edition

  • Published By:
  • ISBN-10: 0080970389
  • ISBN-13: 9780080970387
  • DDC: 616.0756
  • Grade Level Range: College Freshman - College Senior
  • 1036 Pages | eBook
  • Original Copyright 2013 | Published/Released January 2014
  • This publication's content originally published in print form: 2013

  • Price:  Sign in for price



The fourth edition of The Immunoassay Handbook is a thoroughly updated guide to the science, technology, and applications of ELISA and other immunoassays. It describes a wide range of methods and gives insight into the latest developments and applications in clinical and veterinary practice and pharmaceutical and life science research. Revised and extensively updated, with more than 30% new material and 77 chapters, it reveals underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included R&D, design engineering, operations, and strategy. He retired in 2012 to focus on his role as editor of The Immunoassay Handbook.

Table of Contents

Front Cover.
Half Title Page.
Title Page.
Copyright Page.
List of Contributors.
About the Editors.
1: Immunoassay Fundamentals.
2: How to Use this Book.
3: Immunoassay for Beginners.
4: Immunoassay Performance Measures.
5: Immunoassay Configurations.
6: Principles of Competitive and Immunometric Assays (Including ELISA).
7: Non-Competitive Immunoassays for Small Molecules—The Anti-Complex and Selective Antibody Systems.
8: Homogeneous Immunoassays.
9: Lateral Flow Immunoassay Systems: Evolution from the Current State of the Art to the Next Generation of Highly Sensitive, Quantitative Rapid Assays.
10: Ambient Analyte Assay.
11: Free Analyte Immunoassay.
12: Qualitative Immunoassay—Features and Design.
13: Detection of Antibodies Relevant to Infectious Disease.
14: Microsphere-Based Multiplex Immunoassays: Development and Applications Using Luminex® xMAP® Technology.
15: Lab-on-a-Chip, Micro- and Nanoscale Immunoassay Systems, and Microarrays.
16: Immunological Biosensors.
17: Surface Plasmon Resonance in Binding Site, Kinetic, and Concentration Analyses.
18: Measurement of Single Protein Molecules Using Digital ELISA.
19: Immunoassay Components.
20: Antibodies.
21: Signal Generation and Detection Systems (Excluding Homogeneous Assays).
22: Separation Systems.
23: Conjugation Methods.
24: Standardization and Calibration.
25: Calibration Curve Fitting.
26: Related Techniques.
27: The Foundations of Immunochemistry.
28: Immunohistochemistry and Immunocytochemistry.
29: Immunoassay Development.
30: Practical Guide to ELISA Development.
31: Method Evaluation—A Practical Guide.
32: Interferences in Immunoassay.
33: Immunoassay Development in the In Vitro Diagnostic Industry.
34: Immunoassay Implementation.
35: Sample Collection, Including Participant Preparation and Sample Handling.
36: Quality Assurance.
37: Point-of-Care Testing.
38: Choosing an Automated Immunoassay System.
39: Immunoassay Troubleshooting Guide.
40: Immunoassay Product Technology.
41: Introduction to Immunoassay Product Technology in Clinical Diagnostic Testing.
42: Market Trends.
43: Lateral Flow and Consumer Diagnostics.
44: Consumer Diagnostics—Clearblue Pregnancy and Fertility Testing.
45: The Triage® System.
46: Stratus® CS Acute Care™ STAT Fluorometric Analyzer.
47: IMx®.
48: AxSYM®.
49: Abbott ARCHITECT® Family of Analyzers.
50: Advia Centaur® XP.
51: Immulite® and Immulite 1000.
52: Immulite® 2000 and Immulite 2000 XPi.
53: Vitros® ECiQ Immunodiagnostic System, Vitros® 3600 Immunodiagnostic System, and Vitros® 5600 Integrated System.
54: Vitros® Immuno-Rate and MicroTip™ Assays.
55: Access 2.
56: DxI 600 and DxI 800.
57: Elecsys® Immunoassay Systems.
58: Prism™.
59: Phadia Laboratory Systems.
60: Immunoassay Applications Other than Clinical Chemistry.
61: Immunoassay Applications in Veterinary Diagnostics.
62: Ligand Binding Assays in Drug Development.
63: Immunoassay Clinical Applications (Human).
64: Clinical Concepts.
65: Thyroid.
66: The Adrenal Cortex.
67: Bone and Calcium Metabolism.
68: Infertility.
69: In Vitro Fertilization and Embryo Transfer (IVF-ET).
70: Hirsutism and Virilization in the Female.
71: Pregnancy.
72: Growth and Growth Hormone Deficiency.
73: Diabetes Mellitus.
74: Hematology.
75: Cardiac Markers.
76: Cancer Markers.
77: Allergy.
78: Autoimmune Disease.
79: Gastrointestinal Tract.
80: Hepatitis.
81: HIV-1 and HIV-2: Causative Agents of AIDS.
82: Viral Diseases.
83: Bacterial Diseases.
84: Parasites and Fungi.
85: Therapeutic Drug Monitoring (TDM).
86: Drugs of Abuse.