Methods in Protein Design, 1st Edition

  • Published By:
  • ISBN-10: 0123946298
  • ISBN-13: 9780123946294
  • DDC: 660.63
  • Grade Level Range: College Freshman - College Senior
  • 520 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013

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This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers methods in protein design and it has chapters on such topics as protein switch engineering by domain insertion, evolution based design of proteins, and computationally designed proteins.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
1: Methods in Enzymology.
2: Computational Design of Novel Protein Binders and Experimental Affinity Maturation.
3: Mining Tertiary Structural Motifs for Assessment of Designability.
4: Computational Methods for Controlling Binding Specificity.
5: Flexible Backbone Sampling Methods to Model and Design Protein Alternative Conformations.
6: OSPREY: Protein Design with Ensembles, Flexibility, and Provable Algorithms.
7: Scientific Benchmarks for Guiding Macromolecular Energy Function Improvement.
8: Molecular Dynamics Simulations for the Ranking, Evaluation, and Refinement of Computationally Designed Proteins.
9: Multistate Protein Design Using Clever and Classy.
10: Using Analyses of Amino Acid Coevolution to Understand Protein Structure and Function.
11: Evolution-Based Design of Proteins.
12: Protein Engineering and Stabilization from Sequence Statistics Variation and Covariation Analysis.
13: Enzyme Engineering by Targeted Libraries.
14: Generation of High-Performance Binding Proteins for Peptide Motifs by Affinity Clamping.
15: Engineering Fibronectin-Based Binding Proteins by Yeast Surface Display.
16: Engineering and Analysis of Peptide-Recognition Domain Specificities by Phage Display and Deep Sequencing.
17: Efficient Sampling of SCHEMA Chimera Families to Identify Useful Sequence Elements.
18: Protein Switch Engineering by Domain Insertion.
19: Design of Chimeric Proteins by Combination of Subdomain-Sized Fragments.
20: α-Helix Mimicry with α/β-Peptides.
Author Index.
Subject Index.
Color Plates.