Proteomic and Metabolomic Approaches to Biomarker Discovery, 1st Edition

  • Published By:
  • ISBN-10: 0123947952
  • ISBN-13: 9780123947956
  • DDC: 610.28
  • Grade Level Range: College Freshman - College Senior
  • 488 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013

  • Price:  Sign in for price



Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce research errors. Disease biomarker discovery is one of the most important areas of research, because identifying reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes. Proteomic and Metabolomic Approaches to Biomarker Discovery is the only publication that covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process. A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research.

Table of Contents

Front Cover.
Half Title Page.
Title Page.
Copyright Page.
List of Contributors.
1: Biomarker Discovery: Study Design and Execution.
2: Proteomic and Mass Spectrometry Technologies for Biomarker Discovery.
3: Tissue Sample Preparation for Proteomic Analysis.
4: Sample Preparation in Global Metabolomics of Biological Fluids and Tissues.
5: Serum and Plasma Collection: Preanalytical Variables and Standard Operating Procedures in Biomarker Research.
6: Current NMR Strategies for Biomarker Discovery.
7: Using Data-Independent Mass Spectrometry to Extend Detectable Dynamic Range without Prior Fractionation.
8: Gas Chromatography/Mass Spectrometry-Based Metabonomics.
9: Liquid Chromatographic Methods Combined with Mass Spectrometry in Metabolomics.
10: Capillary Electrophoresis–Mass Spectrometry for Proteomic and Metabolic Analysis.
11: Current Gel Electrophoresis Approaches to Low-Abundance Protein Marker Discovery.
12: Two-Dimensional Difference in Gel Electrophoresis for Biomarker Discovery.
13: Affinity Targeting Schemes for Biomarker Research.
14: Asp-Selective Microwave-Supported Acid Proteolysis.
15: Sample Depletion, Fractionation, and Enrichment for Biomarker Discovery.
16: Protein and Metabolite Identification.
17: Quantitative Proteomics in Development of Disease Protein Biomarkers.
18: Mass Spectrometry and NMR Spectroscopy–Based Quantitative Metabolomics.
19: Multivariate Analysis for Metabolomics and Proteomics Data.
20: Top-Down Mass Spectrometry for Protein Molecular Diagnostics and Biomarker Discovery.
21: A Role for Protein–Protein Interaction Networks in the Identification and Characterization of Potential Biomarkers.
22: Reverse Phase Protein Microarray Technology: Advances into the Clinical Research Arena.
23: Autoantibodies and Biomarker Discovery.
24: MicroRNAs and Biomarker Discovery.
25: Imaging Mass Spectrometry of Intact Biomolecules in Tissue Sections.
26: Mass Spectrometry–Based Approach for Protein Biomarker Verification.
27: Mass Spectrometry Metabolomic Data Handling for Biomarker Discovery.
28: Analytical Methods and Biomarker Validation.