Laboratory Methods in Cell Biology: Imaging, 1st Edition

  • Published By:
  • ISBN-10: 0124078079
  • ISBN-13: 9780124078079
  • DDC: 571.638
  • Grade Level Range: College Freshman - College Senior
  • 464 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013

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Cell biology spans among the widest diversity of methods in the biological sciences.  From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone.

Table of Contents

Front Cover.
Halt Title Page.
Series Editors.
Title Page.
Copyright Page.
Preface: Laboratory Methods in Cell Biology.
1: Structural Dynamics of Nucleic Acids by Single-Molecule Fret.
2: Visualizing the Location and the Dynamics of Gene Expression in Living Animals through Bioluminescence Imaging.
3: Quality Control Protocol for Confocal Systems.
4: Electron Microscopy of Bone.
5: Immunostaining: Detection of Signaling Protein Location in Tissues, Cells and Subcellular Compartments.
6: Bimolecular Fluorescence Complementation (Bifc) Analysis of Protein–Protein Interaction: How to Calculate Signal-To-Noise Ratio.
7: Immunohistochemical Detection of Neuropeptides/Transmitters in Mammalian Brain.
8: Intravital Imaging of Autoreactive T Cells in Living Animals.
9: Genetically Encoded Click Chemistry for Single-Molecule Fret of Proteins.
10: High-Throughput Methods for the Detection of Protein Overexpression Using Fluorescence Markers.
11: Correlative Microscopy.
12: Stereology of the Lung.
13: Quantitative 3d Cell-Based Assay Performed with Cellular Spheroids and Fluorescence Microscopy.
14: Optimizing Fluorescence Excitation and Detection for Intravital Two-Photon Microscopy.
15: Two-Dimensional Crystallization by Dialysis for Structural Studies of Membrane Proteins by the Cryo-Em Method Electron Crystallography.
16: In situ Hybridization.
17: RNA Imaging: Tracking in Real-Time RNA Transport in Neurons Using Molecular Beacons and Confocal Microscopy.
Volumes in Series.
Color Plate.