Methods of Adipose Tissue Biology Part B, 1st Edition

  • Volume 2
  • Published By:
  • ISBN-10: 0128003367
  • ISBN-13: 9780128003367
  • DDC: 611.01827
  • Grade Level Range: College Freshman - College Senior
  • 344 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013

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This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale.1) The study of adipose tissue goes hand in hand with our global effort to understand and reverse the epidemic of obesity and associated medical complications. 2) Contributors include leading researchers who have made tremendous contributions to our ability to investigate white and brown adipose tissues. 3) The wide variety of experimental approaches detailed within this volume: including the evaluation of adipose tissue biology at the molecular, biochemical, cellular, tissue, and organismal levels.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
1: Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice.
2: 3-D Adipocyte Differentiation and Peri-Adipocyte Collagen Turnover.
3: Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells.
4: Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells.
5: Human Adipose-Derived Stromal/Stem Cell Isolation, Culture, and Osteogenic Differentiation.
6: Analysis of Adipose Tissue Lipid Using Mass Spectrometry.
7: Measurement of Long-Chain Fatty Acid Uptake into Adipocytes.
8: Measurement of the Unfolded Protein Response to Investigate Its Role in Adipogenesis and Obesity.
9: Application of Activity-Based Protein Profiling to Study Enzyme Function in Adipocytes.
10: Measurement of Lipolysis.
11: Measurement of Lipolysis Products Secreted by 3T3-L1 Adipocytes Using Microfluidics.
12: Methods for Performing Lipidomics in White Adipose Tissue.
13: Measuring Respiratory Activity of Adipocytes and Adipose Tissues in Real Time.
14: Detecting Protein Carbonylation in Adipose Tissue and in Cultured Adipocytes.
15: Use of Fluorescence Microscopy to Probe Intracellular Lipolysis.
16: Evaluation of Protein Phosphorylation during Adipogenesis.
Author Index.
Subject Index.