Cilia, Part A, 1st Edition

  • Volume 1
  • Published By:
  • ISBN-10: 0123984866
  • ISBN-13: 9780123984869
  • DDC: 571.67
  • Grade Level Range: College Freshman - College Senior
  • 472 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013

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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.

Table of Contents

Front Cover.
Half Title Page.
Other Front Matter.
Title Page.
Copyright Page.
Methods in Enzymology.
1: Isolation of Intraflagellar Transport Particle Proteins from Chlamydomonas Reinhardtii.
2: Molecular Tools for Studying the Radial Spoke.
3: Methods for Analysis of Calcium/Calmodulin Signaling in Cilia and Flagella.
4: Analysis of Flagellar Protein Ubiquitination.
5: Analysis of Ciliary Import.
6: Analysis of Signaling Pathways Controlling Flagellar Movements in Mammalian Spermatozoa.
7: Analysis of Microtubule Plus-End-Tracking Proteins in Cilia.
8: Biochemical and Physiological Analysis of Axonemal Dyneins.
9: Measuring the Regulation of Dynein Activity During Flagellar Motility.
10: Analysis of Interactions between Intraflagellar Transport Proteins.
11: Analysis of Ciliary Membrane Protein Dynamics Using SNAP Technology.
12: Engineering Oscillating Microtubule Bundles.
13: Purification of Exosome-Like Vesicles from Urine.
14: Electron Microscopy of Flagella, Primary Cilia, and Intraflagellar Transport in Flat-Embedded Cells.
15: In vivo Imaging of IFT in Chlamydomonas Flagella.
16: Measuring Rates of Intraflagellar Transport Along Caenorhabditis elegans Sensory Cilia Using Fluorescence Microscopy.
17: 3D Structural Analysis of Flagella/Cilia by Cryo-Electron Tomography.
18: Electron Tomography of IFT Particles.
19: Reconstitution of Flagellar Sliding.
Color Plate.
Author Index.
Subject Index.