Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate, 1st Edition

  • Published By:
  • ISBN-10: 012420094X
  • ISBN-13: 9780124200944
  • DDC: 572.7
  • Grade Level Range: College Freshman - College Senior
  • 408 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013

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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
Methods in Enzymology.
1: Cell Protocols: Basic Microbiological Techniques.
2: Pouring Agar Plates and Streaking or Spreading to Isolate Individual Colonies.
3: Storage of Bacteria and Yeast.
4: Cell Protocols: Cellular Fractionation.
5: Cellular Fractionation – Mammalian Cells.
6: Cellular Fractionation – Yeast Cells.
7: Cell Protocols: Knock-outs and Gene.
8: Yeast-Gene Replacement Using PCR Products.
9: Measuring RNAi Knockdown using qPCR.
10: Recombineering: Using Drug Cassettes to Knock out Genes in vivo.
11: Gene Knockouts, in vivo Site-Directed Mutagenesis and Other Modifications Using the Delitto Perfetto System in Saccharomyces cerevisiae.
12: Gene Targeting and Site-Specific Recombination in Mouse ES Cells.
13: Recombineering Highly Efficient in vivo Genetic Engineering using Single-strand Oligos.
14: Cell Protocols: Microbiological Media.
15: Growth Media for E. coli.
16: Saccharomyces Cerevisiae Growth Media.
17: Cell Protocols: Preparation of Slides for Microscopy.
18: Immunohistochemistry on Freely Floating Fixed Tissue Sections.
19: Preparation of Slides for Microscopy from Frozen Tissue Sections.
20: Preparation of Formalin-fixed Paraffin-embedded Tissue for Immunohistochemistry.
21: Preparation of Cells for Microscopy using Cytospin.
22: Preparation of Cells for Microscopy using Chamber Slides and Coverslips.
23: Preparation of Cells for Microscopy using ‘Cell Blocks’.
24: Lipid and Carbohydrate Protocols.
25: Enzymatic Deglycosylation of Glycoproteins.
26: Lipid and Carbohydrate Protocol: Making Vesicles and Micelles.
27: Preparation of Fatty Acid or Phospholipid Vesicles by Thin-film Rehydration.
28: Vesicle Extrusion Through Polycarbonate Track-etched Membranes using a Hand-held Mini-extruder.
29: Preparation of Fatty Acid Micelles.
30: Other Protocols.
31: Reverse-phase HPLC Analysis and Purification of Small Molecules.
32: Thin Layer Chromatography.
33: ATP and GTP Hydrolysis Assays (TLC).
Author Index.
Subject Index.