G Protein Coupled Receptors, 1st Edition

  • Volume 521
  • Published By:
  • ISBN-10: 0123918731
  • ISBN-13: 9780123918734
  • DDC: 572.696
  • Grade Level Range: College Freshman - College Senior
  • 480 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013

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This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high throughput assays for identification of pharmacoperone drugs.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
Methods in Enzymology.
1: GPCR Trafficking.
2: Therapeutic Rescue of Misfolded/Mistrafficked Mutants: Automation-Friendly High-Throughput Assays for Identification of Pharmacoperone Drugs of GPCRs.
3: Trafficking of the Follitropin Receptor.
4: Single-Molecule Imaging Technique to Study the Dynamic Regulation of GPCR Function at the Plasma Membrane.
5: GPCR Oligomerization and Receptor Trafficking.
6: β-Arrestins and G Protein-Coupled Receptor Trafficking.
7: Tracking Cell Surface Mobility of GPCRs Using α-Bungarotoxin-Linked Fluorophores.
8: Regulatory Mechanism of G Protein-Coupled Receptor Trafficking to the Plasma Membrane: A Role for mRNA Localization.
9: Dissecting Trafficking and Signaling of Atypical Chemokine Receptors.
10: Trafficking Motifs.
11: Systematic and Quantitative Analysis of G Protein-Coupled Receptor Trafficking Motifs.
12: Identification of Endoplasmic Reticulum Export Motifs for G Protein-Coupled Receptors.
13: Amino Acid Residues of G-Protein-Coupled Receptors Critical for Endoplasmic Reticulum Export and Trafficking.
14: GPCR Oligomerization.
15: G-Protein-Coupled Heteromers:Regulation in Disease.
16: Hetero-oligomerization and Specificity Changes of G Protein-Coupled Purinergic Receptors Novel Insight into Diversification of Signal Transduction.
17: Bimolecular Fluorescence Complementation Analysis of G Protein-Coupled Receptor Dimerization in Living Cells.
18: G Protein–Coupled Receptor Heterodimerization in the Brain.
19: Experimental Strategies for Studying G Protein-Coupled Receptor Homo- and Heteromerization with Radioligand Binding and Signal Transduction Methods.
20: Analysis of GPCR Dimerization Using Acceptor Photobleaching Resonance Energy Transfer Techniques.
21: Techniques for the Discovery of GPCR-Associated Protein Complexes.
22: Expression, Purification, and Analysis of G-Protein-Coupled Receptor Kinases.
23: Modern Methods to Investigate the Oligomerization of Glycoprotein Hormone Receptors (TSHR, LHR, FSHR).
Color Plate.
Author Index.
Subject Index.