Methods for analysis of Golgi complex function, 1st Edition

  • Published By:
  • ISBN-10: 0124171834
  • ISBN-13: 9780124171831
  • DDC: 571.656
  • Grade Level Range: College Freshman - College Senior
  • 498 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013

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This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies. Chapters are written by experts in the field Cutting-edge material.

Table of Contents

Front Cover.
Half Title Page.
Other Front Matter.
Title Page.
Copyright Page.
1: Model Systems and Functional Studies.
2: Analysis of GOLGI Complex Functions: In Vitro Reconstitution Systems.
3: RNA Interference Approaches to Examine GOLGI Function in Animal Cell Culture.
4: Trafficking along the Secretory Pathway in Drosophila Cell Line and Tissues: A Light and Electron Microscopy Approach.
5: Studying Membrane Trafficking in the Worm C elegans by RNA Interference.
6: Endomembrane and GOLGI Traffic in Plant Cells.
7: Biochemical Analysis of Secretory Trafficking in Mammalian Cells.
8: Methods for Analysis of Apical Lumen Trafficking Using Micropatterned 3d Systems.
9: Using Replication Defective Viruses to Analyze Membrane Trafficking in Polarized Epithelial Cells.
10: Snap-Tagging the Retrograde Route.
11: Assessing ER and GOLGI N-Glycosylation Process Using Metabolic Labeling in Mammalian Cultured Cells.
12: Imaging-Based Approaches.
13: Fluorescence-Based Analysis of Trafficking in Mammalian Cells.
14: Photobleaching Methods to Study GOLGI Complex Dynamics in Living Cells.
15: Photohighlighting Approaches to Access Membrane Dynamics of the GOLGI Apparatus.
16: Live Cell Visualization of GOLGI Membrane Dynamics by Super-Resolution Confocal Live Imaging Microscopy.
17: Analysis of GOLGI Complex Function Using Correlative Light-Electron Microscopy.
18: Viewing Golgi Structure and Function from a Different Perspective—insights from Electron Tomography.
19: High-Content Screening and Analysis of the GOLGI Complex.
20: Emerging Studies [New Frontiers].
21: (Re)Modeling the GOLGI.
22: Golgi Depletion from Living Cells with Laser Nanosurgery.
23: Studying Intracellular Trafficking Pathways with Probabilistic Density Maps.
24: Methods to Study Signaling at the GOLGI Apparatus.
25: Golgi-Dependent Signaling: Self-Coordination of Membrane Trafficking.
26: Signaling at the GOLGI during Mitosis.
27: Ice Recovery Assay for Detection of GOLGI-Derived Microtubules.
Volumes in Series.
Color Plate.