Methods of Adipose Tissue Biology Part A, 1st Edition

  • Volume 1
  • Published By:
  • ISBN-10: 012411606X
  • ISBN-13: 9780124116061
  • DDC: 611.01827
  • Grade Level Range: College Freshman - College Senior
  • 360 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013

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Methods of Adipose Tissue Biology is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers who are world leaders in this field. Detailed lab protocols include methods to visualize adipocytes and adipose tissues in humans and experimental models, converting stem cells into white and brown adipocytes in vitro, evaluating aspects of adipocyte metabolism, inducibly knocking out genes in adipose tissues, and evaluating transcriptional control of adipogenesis on a global scale.

Table of Contents

Front Cover.
Half Title Page.
Other Front Matter.
Title Page.
Copyright Page.
1: Adipocyte-Specific Transgenic and Knockout Models.
2: Imaging White Adipose Tissue with Confocal Microscopy.
3: Isolation and Study of Adipocyte Precursors.
4: Imaging of Adipose Tissue.
5: Adipose Tissue Angiogenesis Assay.
6: Quantifying Size and Number of Adipocytes in Adipose Tissue.
7: Use of Osmium Tetroxide Staining with Microcomputerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue In Vivo.
8: Brown Adipose Tissue in Humans Detection and Functional Analysis Using PET (Positron Emission Tomography), MRI (Magnetic Resonance Imaging), and DECT (Dual Energy Computed Tomography).
9: Analyzing the Functions and Structure of the Human Lipodystrophy Protein Seipin.
10: Differentiation of Human Pluripotent Stem Cells into Highly Functional Classical Brown Adipocytes.
11: Analysis and Measurement of the Sympathetic and Sensory Innervation of White and Brown Adipose Tissue.
12: Measurement and Manipulation of Human Adipose Tissue Blood Flow Using Xenon Washout Technique and Adipose Tissue Microinfusion.
13: Isolation and Quantitation of Adiponectin Higher Order Complexes.
14: Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by Chip-Seq.
15: Analysis and Isolation of Adipocytes by Flow Cytometry.
16: Flow Cytometry Analyses of Adipose Tissue Macrophages.
Other Back Matter.
Author Index.
Subject Index.