Digital Microscopy, 4th Edition

  • Published By:
  • ISBN-10: 0124078923
  • ISBN-13: 9780124078925
  • DDC: 570.282
  • Grade Level Range: College Freshman - College Senior
  • 672 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013

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The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe?s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.

Table of Contents

Front Cover.
Half Title Page.
Series Editors.
Title Page.
Copyright Page.
1: Microscope Basics.
2: The Optics of Microscope Image Formation.
3: Proper Alignment of the Microscope.
4: Fundamentals of Fluorescence and Fluorescence Microscopy.
5: Fluorescent Protein Applications in Microscopy.
6: Live-Cell Fluorescence Imaging.
7: Practical Aspects of Adjusting Digital Cameras.
8: Cameras for Digital Microscopy.
9: A High-Resolution Multimode Digital Microscope System.
10: Electronic Cameras for Low-Light Microscopy.
11: Camera Technologies for Low Light Imaging: Overview and Relative Advantages.
12: Post-Processing for Statistical Image Analysis in Light Microscopy.
13: 65,000 Shades of Grey: Use of Digital Image Files in Light Microscopy.
14: Quantitative Analysis of Digital Microscope Images.
15: Evaluating Optical Aberrations Using Fluorescent Microspheres: Methods, Analysis, and Corrective Actions.
16: Ratio Imaging: Practical Considerations for Measuring Intracellular Ca2+ and pH in Living Cells.
17: Quantitative Fluorescence Microscopy and Image Deconvolution.
18: Practical Aspects of Quantitative Confocal Microscopy.
19: Theoretical Principles and Practical Considerations for Fluorescence Resonance Energy Transfer Microscopy.
20: Fluorescence Lifetime Imaging Microscopy for Quantitative Biological Imaging.
21: Fluorescence Correlation Spectroscopy: Molecular Complexing in Solution and in Living Cells.
22: Breaking the Resolution Limit in Light Microscopy.
23: Circumventing Photodamage in Live-Cell Microscopy.
24: A User's Guide to Localization-Based Super-Resolution Fluorescence Imaging.
25: Quantitative Ratiometric Imaging of FRET-Biosensors in Living Cells.
26: Tip-Enhanced Raman Spectroscopy for the Base Interrogation of DNA.
Volumes in Series.
Color Plate.