eBook G Protein Coupled Receptors, 1st Edition

  • Volume 520
  • Published By:
  • ISBN-10: 0123918723
  • ISBN-13: 9780123918727
  • DDC: 572.696
  • Grade Level Range: College Freshman - College Senior
  • 440 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013
  • Price:  Sign in for price

About

Overview

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
Contents.
Contributors.
Preface.
Methods in Enzymology.
1: Expression of GPCRs in Pichia Pastoris for Structural Studies.
2: Conformational Ensemble View of G Protein-Coupled Receptors and the Effect of Mutations and Ligand Binding.
3: Structural Evolution of G-Protein-Coupled Receptors.
4: Directed Evolution of G-Protein-Coupled Receptors for High Functional Expression and Detergent Stability.
5: The Role of Hydrophobic Amino Acids in the Structure and Function of the Rhodopsin Family of G Protein-Coupled Receptors.
6: Structure of β-Adrenergic Receptors.
7: Advances in Methods to Characterize Ligand-Induced Ionic Lock and Rotamer Toggle Molecular Switch in G Protein-Coupled Receptors.
8: Crystallogenesis of Adenosine A2A Receptor—T4 Lysozyme Fusion Protein.
9: Probing GPCR Structure.
10: Strategies for Studying the Ligand Binding Site of GPCRs: Photoaffinity Labeling of the VPAC1 Receptor, a Prototype of Class B GPCRs.
11: Expression of Mammalian G Protein-Coupled Receptors in Caenorhabditis elegans.
12: Expression, Purification, and Structural Analysis of Intracellular C-Termini from Metabotropic Glutamate Receptors.
13: Unnatural Amino Acid Mutagenesis of GPCRs Using Amber Codon Suppression and Bioorthogonal Labeling.
14: Mapping a Ligand Binding Site Using Genetically Encoded Photoactivatable Crosslinkers.
15: Alternative mRNA Splicing of G Protein-Coupled Receptors.
16: Functional Residues Essential for the Activation of the CB1 Cannabinoid Receptor.
Author Index.
Subject Index.