eBook Microbial Metagenomics, Metatranscriptomics, and Metaproteomics, 1st Edition

  • Published By:
  • ISBN-10: 012407927X
  • ISBN-13: 9780124079274
  • DDC: 572.8629
  • Grade Level Range: College Freshman - College Senior
  • 688 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013
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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers microbial metagenomics, metatranscriptomics, and metaproteomics, and includes chapters on such topics as in-solution FISH for single cell genome preparation, preparation of BAC libraries from marine microbial community DNA, and preparation of microbial community cDNA for metatranscriptomic analysis in marine plankton.

Table of Contents

Front Cover.
Half Title Page.
Other Front Matter.
Title Page.
Copyright Page.
Methods In Enzymology.
1: Preparation and Genomic Analyses of Single Cells from Natural Populations.
2: In-solution Fluorescence In Situ Hybridization and Fluorescence-activated Cell Sorting for Single Cell and Population Genome Recovery.
3: Whole Cell Immunomagnetic Enrichment of Environmental Microbial Consortia Using Rrna-targeted Magneto-fish.
4: Coupling FACS and Genomic Methods for the Characterization of Uncultivated Symbionts.
5: Optofluidic Cell Selection from Complex Microbial Communities for Single-genome Analysis.
6: Quantifying and Identifying the Active and Damaged Subsets of Indigenous Microbial Communities.
7: Microbial Community Genomics—sampling, Metagenomic Library Preparation, and Analysis.
8: Preparation of BAC Libraries from Marine Microbial Populations.
9: Preparation of Fosmid Libraries and Functional Metagenomic Analysis of Microbial Community DNA.
10: Preparation of Metagenomic Libraries from Naturally Occurring Marine Viruses.
11: Microbial Community Transcriptomics—sample Preparation, Cdna Library Construction, and Analysis.
12: Preparation and Metatranscriptomic Analyses of Host–microbe Systems.
13: Preparation of Microbial Community Cdna for Metatranscriptomic Analysis in Marine Plankton.
14: Sequential Isolation of Metabolites, RNA, DNA, and Proteins from the Same Unique Sample.
15: Use of Internal Standards for Quantitative Metatranscriptome and Metagenome Analysis.
16: Sample Processing and Cdna Preparation for Microbial Metatranscriptomics in Complex Soil Communities.
17: Microbial Community Proteomics—sampling, Sample Preparation, Spectral Analysis, and Interpretation.
18: Sample Preparation and Processing for Planktonic Microbial Community Proteomics.
19: Sample Handling and Mass Spectrometry for Microbial Metaproteomic Analyses.
20: Molecular Tools for Investigating Microbial Community Structure and Function in Oxygen-deficient Marine Waters.
21: Microbial Community Informatics: Computational Platforms, Comparative Analyses, and Statistics.
22: Assembling Full-length Rrna Genes from Short-read Metagenomic Sequence Datasets Using EMIRGE.
23: Computational Methods for High-throughput Comparative Analyses of Natural Microbial Communities.
24: Advancing Our Understanding of the Human Microbiome Using QIIME.
25: Disentangling Associated Genomes.
26: Microbial Community Analysis Using MEGAN.
27: A Metagenomics Portal for a Democratized Sequencing World.
28: A User’s Guide to Quantitative and Comparative Analysis of Metagenomic Datasets.
Author Index.
Subject Index.