eBook Hydrogen Peroxide and Cell Signaling, Part C, 1st Edition

  • Volume 3
  • Published By:
  • ISBN-10: 0124055400
  • ISBN-13: 9780124055407
  • DDC: 571.6
  • Grade Level Range: College Freshman - College Senior
  • 384 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013
  • Price:  Sign in for price

About

Overview

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy.

Table of Contents

Front Cover.
Half Title Page.
Series Page.
Title Page.
Copyright Page.
Contents.
Contributors.
Preface.
Methods in Enzymology.
1: H2O2 Regulation of Cell Signaling.
2: The Biological Chemistry of Hydrogen Peroxide.
3: Reactive Oxygen Species in the Activation of map Kinases.
4: Hydrogen Peroxide Signaling Mediator in the Activation of P38 MAPK in Vascular Endothelial Cells.
5: In Vivo Imaging of Nitric Oxide and Hydrogen Peroxide in Cardiac Myocytes.
6: Methods for Studying Oxidative Regulation of Protein Kinase C.
7: P66shc, Mitochondria, and the Generation of Reactive Oxygen Species.
8: Detecting Disulfide-Bound Complexes and the Oxidative Regulation of Cyclic Nucleotide-Dependent Protein Kinases by H2O2.
9: Redox Regulation of Protein Tyrosine Phosphatases: Methods for Kinetic Analysis of Covalent Enzyme Inactivation.
10: H2O2 in the Redox Regulation of Transcription and Cell-Surface Receptors.
11: Chapter Nine: Activation of Nrf2 By H2O2 De Novo Synthesis versus Nuclear Translocation.
12: Chapter Ten: H2O2 in the Induction of Nf-κb-dependent Selective Gene Expression.
13: Chapter Eleven: Detection of H2O2-Mediated Phosphorylation of Kinase-Inactive Pdgfrα.
14: H2O2 and Regulation of Cellular Processes.
15: Chapter Twelve: Genetic Modifier Screens to Identify Components of a Redox-Regulated Cell Adhesion and Migration Pathway.
16: Investigating the Role of Reactive Oxygen Species in Regulating Autophagy.
17: H2O2 a Chemoattractant?.
18: Measuring Mitochondrial Uncoupling Protein-2 Level and Activity in Insulinoma Cells.
19: Effects of H2O2 on Insulin Signaling the Glucose Transport System in Mammalian Skeletal Muscle.
20: Monitoring of Hydrogen Peroxide and Other Reactive Oxygen and Nitrogen Species Generated by Skeletal Muscle.
Author Index.
Subject Index.
Color Plate.