eBook Endosome Signalling Part B, 1st Edition

  • Volume 2
  • Published By:
  • ISBN-10: 0123984815
  • ISBN-13: 9780123984814
  • DDC: 571.655
  • Grade Level Range: College Freshman - College Senior
  • 518 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013
  • Price:  Sign in for price



This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the second of two volumes on endosome signaling and includes chapters on such topics as measurement of entry into the endosomal compartment by multi-parametric image analysis, assessment of peptide internalization and endosomal signaling, and VEGF-A in endosomal signaling.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
1: Signaling.
2: Assessment of Insulin Proteolysis in Rat Liver Endosomes: Its Relationship to Intracellular Insulin Signaling.
3: A Bimolecular Fluorescent Complementation Screen Reveals Complex Roles of Endosomes in Ras-Mediated Signaling.
4: TGFβ in Endosomal Signaling.
5: Annexins and Endosomal Signaling.
6: Analysis, Regulation, and Roles of Endosomal Phosphoinositides.
7: Mild Fixation and Permeabilization Protocol for Preserving Structures of Endosomes, Focal Adhesions, and Actin Filaments during Immunofluorescence Analysis.
8: Characterizing and Measuring Endocytosis of Lipid-Binding Effectors in Mammalian Cells.
9: Measuring the Role for Met Endosomal Signaling in Tumorigenesis.
10: Intracellular Toll-Like Receptor Recruitment and Cleavage in Endosomal/Lysosomal Organelles.
11: Assessment of the Toll-Like Receptor 3 Pathway in Endosomal Signaling.
12: Labeling of Platelet-Derived Growth Factor by Reversible Biotinylation to Visualize Its Endocytosis by Microscopy.
13: Endosomal Signaling and Oncogenesis.
14: Ros-Containing Endosomal Compartments Implications for Signaling.
15: SARA and RNF11 at the Crossroads of EGFR Signaling and Trafficking.
16: p18/LAMTOR1: A LAte Endosome/Lysosome-Specific Anchor Protein for the mTORC1/MAPK SignAling PAthwAy.
17: Vascular Endothelial Growth Factor A-Stimulated Signaling from Endosomes in Primary Endothelial Cells.
18: Assessment of Internalization and Endosomal Signaling: Studies with Insulin and EGF.
19: Quantitative Proteomic Analysis of Compartmentalized Signaling Networks.
20: Separation of Magnetically Isolated TNF Receptosomes from Mitochondria.
21: Deubiquitinases and Their Emerging Roles in β-Arrestin-mediated Signaling.
22: TSLP Expression Induced via Toll-Like Receptor Pathways in Human Keratinocytes.
23: Endosomal Signaling by Protease-Activated Receptors.
24: Investigating Signaling Consequences of GPCR Trafficking in the Endocytic Pathway.
25: MICAL-Like1 in Endosomal Signaling.
Other Backmatter.
Author Index.
Subject Index.