eBook Comprehensive Biophysics, 1st Edition

  • Edward Egelman
  • Published By:
  • ISBN-10: 0080957188
  • ISBN-13: 9780080957180
  • DDC: 571.4
  • Grade Level Range: College Freshman - College Senior
  • 3524 Pages | eBook
  • Original Copyright 2012 | Published/Released August 2013
  • This publication's content originally published in print form: 2012
  • Price:  Sign in for price

About

Overview

Unites the different areas of biophysical research and allows users to navigate through the most essential concepts with ease.

Table of Contents

Front Cover.
Half Title Page.
Title Page.
Copyright Page.
Contents.
Editor-in-Chief.
Editorial Board.
Contributors to Volume 1.
Contents of All Volumes.
Preface.
1: Volume 1 Biophysical Techniques for Structural Characterization of Macromolecules.
2: Volume Introduction.
3: Overview and Historical.
4: Cantor and Schimmel – 30 Years Later.
5: Protein Production Strategies for High-Throughput Structure Determination.
6: Efficient Strategies for Production of Eukaryotic Proteins.
7: X-Ray Crystallography.
8: X-Ray Crystallography: Crystallization.
9: X-Ray Crystallography: Data Collection Strategies and Resources.
10: Phasing of X-Ray Data.
11: Refinement of X-Ray Crystal Structures.
12: Structure Validation and Analysis.
13: NMR Spectroscopy.
14: Introduction to Solution State NMR Spectroscopy.
15: Solid State NMR Methods.
16: The Hybrid Solution/Solid-State NMR Method for Membrane Protein Structure Determination.
17: Labeling Techniques.
18: NMR Spectroscopy: NMR Relaxation Methods.
19: Electron Microscopy.
20: Structure Determination of Macromolecular Complexes by Cryo-Electron Microscopy in vitro and in situ.
21: Analysis of 2-D Crystals of Membrane Proteins by Electron Microscopy.
22: Cryo-Electron Microscopy and Tomography of Virus Particles.
23: Mass Spectrometry.
24: Mass Spectrometry.
25: SAXS.
26: Small and Wide Angle X-Ray Scattering from Biological Macromolecules and Their Complexes in Solution.
27: Ultrafast Spectroscopic Techniques.
28: Ultrafast Structural Dynamics of Biological Systems.
29: EPR and Other Electron Spectroscopies.
30: Electron Magnetic Resonance.
31: Computation.
32: Computation of Structure, Dynamics, and Thermodynamics of Proteins.
33: Fast Flow.
34: Rapid Mixing Techniques for the Study of Enzyme Catalysis.
35: Other Spectroscopy – UV-Vis, CD, Raman, Vibrational CD Applied in Biophysical Research.
36: Optical Spectroscopy.
37: FRET/Fluorescence.
38: Fluorescence and FRET: Theoretical Concepts 101.
Half Title Page.
Title Page.
Copyright Page.
Contents.
Editor-in-Chief.
Editorial Board.
Contributors to Volume 2.
Contents of All Volumes.
Preface.
1: Volume 2 Biophysical Techniques for Characterization of Cells.
2: Volume Introduction.
3: Elucidating Cellular Structures.
4: Confocal Microscopy.
5: Fluorescence Lifetime Microscopy: The Phasor Approach.
6: Super-Resolution Microscopy.
7: Studying the Macromolecular Machinery of Cells in situ by Cryo-Electron Tomography.
8: Visualizing Sub-Cellular Organization Using Soft X-Ray Tomography.
9: Atomic Force Microscopy.
10: Super-Resolution Near-Field Optical Microscopy.
11: CARS Microscopy.
12: Elucidating Cellular Dynamics.
13: Quantitative Fluorescent Speckle Microscopy.
14: Fluorescence Correlation Spectroscopy.
15: Image Correlation Spectroscopy.
16: The Basics and Potential of Single-Molecule Tracking in Cellular Biophysics.
Half Title Page.
Title Page.
Copyright Page.
Contents.
Editor-in-Chief.
Editorial Board.
Contributors to Volume 3.
Contents of All Volumes.
Preface.
1: Volume 3 The Folding of Proteins and Nucleic Acids: Protein Folding.
2: Combining Simulation and Experiment to Map Protein Folding.
3: Globular Proteins.
4: Energetics of Protein Folding.
5: Fast Events in Protein Folding.
6: Intermediates in Protein Folding.
7: Characterization of the Denatured State.
8: Single-Molecule Spectroscopy of Protein Folding.
9: Simulation Studies of Force-Induced Unfolding.
10: Protein and Nucleic Acid Folding: Domain Swapping in Proteins.
11: Intrinsically Disordered Proteins.
12: Chaperones and Protein Folding.
13: Protein Switches.
14: Repeat/Non-Globular Proteins.
15: The Folding of Repeat Proteins.
16: Membrane Proteins.
17: The Membrane Factor: Biophysical Studies of Alpha Helical Transmembrane Protein Folding.
18: Nucleic Acid Folding.
19: Effect of Protein Binding on RNA Folding.
Half Title Page.
Title Page.
Copyright Page.
Contents.
Editor-in-Chief.
Editorial Board.
Contributors to Volume 4.
Contents of All Volumes.
Preface.
1: Volume 4 Molecular Motors and Motility.
2: Introduction.
3: General Theoretical Considerations.
4: Microscopic Reversibility and Free-Energy Transduction by Molecular Motors and Pumps.
5: Actin.
6: Structure and Dynamic States of Actin Filaments.
7: Actin Filament Nucleation and Elongation.
8: Mechanical Properties of Actin Networks.
9: Microtubles.
10: Tubulin and Microtubule Structure: Mechanistic Insights into Dynamic Instability and Its Biological Relevance.
11: Force Generation by Dynamic Microtubule Polymers.
12: Myosin.
13: Myosin Motors: Structural Aspects and Functionality.
14: Myosin Motors: Kinetics of Myosin.
15: Single Molecule Fluorescence Techniques for Myosin.
16: Muscle.
17: Cell-Based Studies of the Molecular Mechanism of Muscle Contraction.
18: Spectroscopic Probes of Muscle Proteins.
19: Thin Filament Regulation.
20: Smooth Muscle and Myosin Regulation.
21: Non-Muscle Motility.
22: Intracellular Transport: Relating Single-Molecule Properties to In Vivo Function.
23: Mechanical Forces in Mitosis.
24: Kinesin.
25: Kinesin Structure and Biochemistry.
26: Kinesin Single-Molecule Mechanics.
27: Dynein.
28: Cytoplasmic Dynein: Its ATPase Cycle and ATPase-Dependent Structural Changes.
29: Axonemal Motility.
30: Nucleic Acid Motors.
31: The Ribosome.
32: Viral DNA Packaging Motors.
Half Title Page.
Title Page.
Copyright Page.
Table of Contents.
Editor-in-Chief.
Editorial Board.
Contributors to Volume 5.
Contents of All Volumes.
Preface.
1: Volume 5 Membranes: Volume Introduction.
2: Biophysics of Membranes.
3: Lipid Bilayers.
4: Lipid Bilayer Structure.
5: Membrane Domains and Their Relevance to the Organization of Biological Membranes.
6: Atomic Force Microscopy and Fluorescence Microscopy of Lipid Bilayers.
7: Detergent Interactions with Lipid Bilayers and Membrane Proteins.
8: Membrane Proteins.
9: Atomic Force Microscopy and Electron Microscopy of Membrane Proteins.
10: Solution NMR Spectroscopy of Integral Membrane Proteins.
11: Structure and Folding of Outer Membrane Proteins.
12: Pore-Forming Toxins.
13: Protein Interactions with Membranes.
14: Interactions of Antimicrobial Peptides with Lipid Bilayers.
15: Membrane Recruitment of Signaling Domains.
16: Membrane Protein–Lipid Match and Mismatch.
17: Supported Membranes – Structure and Interactions.
18: Membrane Fusion.
19: The Biophysics of Membrane Fusion.
20: Mechanisms of Enveloped Virus Entry by Membrane Fusion.
21: Membrane Dynamics.
22: Computer Simulation of Membrane Dynamics.
23: Single Molecule Measurements in Membranes.
Half Title Page.
Title Page.
Copyright Page.
Contents.
Editor-in-Chief.
Editorial Board.
Contributors to Volume 6.
Contents of All Volumes.
Preface.
1: Volume 6 Channels.
2: Channel Proteins – An Introduction.
3: Structure-Function Correlates of Glutamate-Gated Ion Channels.
4: Gating Dynamics of the Potassium Channel Pore.
5: Biophysics of TRP Channels.
6: Mechanosensory Transduction.
7: Structures and Mechanisms in Chloride Channels.
8: Biophysics of Ceramide Channels.
9: Voltage Gated Proton Channels.
10: STIM1-ORAI1 Store-Operated Calcium Channels.
11: Structure–Function Correlates in Plant Ion Channels.
Half Title Page.
Title Page.
Copyright Page.
Contents.
Editor-in-Chief.
Editorial Board.
Contributors to Volume 7.
Contents of All Volumes.
Preface.
1: Volume 7 Cell Biophysics.
2: Introduction.
3: Biophysics of Cell-Matrix Adhesion.
4: Biophysics of Selectin-Mediated Cell Adhesion.
5: Biophysics of Cadherin-Mediated Cell–Cell Adhesion.
6: Understanding How Dividing Cells Change Shape.
7: Biophysics of Bacterial Cell Growth and Division.
8: Biophysics of Three-Dimensional Cell Motility.
9: Biophysics of Molecular Cell Mechanics.
10: Biophysics of Nuclear Organization and Dynamics.
11: Cell-Extracellular Matrix Mechanobiology in Cancer.
12: Biomechanics of Cell Motility.
13: Biophysics of Cell Developmental Processes: A Lasercutter’s Perspective.
14: Bacterial Organization in Space and Time.
Half Title Page.
Title Page.
Copyright Page.
Contents.
Editor-in-Chief.
Editorial Board.
Contributors to Volume 8.
Contents of All Volumes.
Preface.
1: Volume 8 Bioenergetics.
2: Ion Electrochemical Gradients, Roles and Measurements.
3: Structure-Function Relationships in P-Type ATPases.
4: Rotational Catalysis by F1-ATPase.
5: The Rotary Bacterial Flagellar Motor.
6: Electron Transfer Chains: Structures, Mechanisms and Energy Coupling.
7: Light Capture in Photosynthesis.
8: The Structure-Function Relationships of Photosynthetic Reaction Centers.
9: Molecular Aspects of the Translocation Process by ABC Proteins.
10: Structural and Mechanistic Aspects of Mitochondrial Transport Proteins.
11: Light Capture and Energy Transduction in Bacterial Rhodopsins and Related Proteins.
12: Transporters and Co-Transporters in Theory and Practice.
13: Membrane Proteins for Secondary Active Transport and Their Molecular Mechanisms.
Half Title Page.
Title Page.
Copyright Page.
Contents.
Editor-in-Chief.
Editorial Board.
Contributors to Volume 9.
Contents of All Volumes.
Preface.
1: Volume 9 Simulation and Modeling: Volume Introduction.
2: Theoretical Biophysics: A Cornerstone of Understanding in Modern Biology and Biomedicine.
3: In Silico Approaches to Structures and Function of Cell Components and Their Aggregates.
4: Coarse-Grained Methods: Theory.
5: In Silico Coarse-Grained Approaches to Structural Dynamics and Function of Proteins and Their Assemblies.
6: Coarse Grained Methods: Applications to Membranes.
7: Dynamics of Very Large Systems: The Ribosome.
8: New Technologies for Molecular Dynamics Simulations.
9: Simulations of Molecular Mechanisms.
10: Molecular Modeling and Simulations of Transporter Proteins – The Transmembrane Allosteric Machinery.
11: Gene Protein Coupled Receptors.
12: Quantum Mechanical Methods for Enzyme Modeling: Accurate Computation of Kinetic Isotope Effects.
13: MNDO-PSDCI and the Analysis of the Photophysical Properties of Visual Chromophores and Retinal Proteins.
14: In Silico Approaches to Structure and Function of Cell Components and Their Assemblies.
15: Molecular Electrostatics and Solvent Effects.
16: Interactions of the Cell Membrane with Integral Proteins.
17: Modeling of Interaction Networks in the Cell.
18: Theory and Mathematical Methods.
19: Mathematical Modeling of Complex Biological Systems.
20: From Genes and Molecules to Organs and Organisms: Heart.
21: Biophysical Representation of Kidney Function.
22: Systems Immunology: A Primer for Biophysicists.
Index.