eBook Fluorescence Fluctuation Spectroscopy (FFS), Part A, 1st Edition

  • Volume 1
  • Published By:
  • ISBN-10: 0123884233
  • ISBN-13: 9780123884237
  • DDC: 535.352
  • Grade Level Range: College Freshman - College Senior
  • 336 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013
  • Price:  Sign in for price



This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation SpectroscopyContains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.

Table of Contents

Front Cover.
Half Title Page.
Title Page.
Copyright Page.
Methods in Enzymology.
1: 40 Years of FCS: How It All Began.
2: Brief Introduction to Fluorescence Correlation Spectroscopy.
3: Dual-Color Fluorescence Cross-Correlation Spectroscopy with Continuous Laser Excitation in a Confocal Setup.
4: Brightness Analysis.
5: Time-Integrated Fluorescence Cumulant Analysis and Its Application in Living Cells.
6: Raster Image Correlation Spectroscopy and Number and Brightness Analysis.
7: Global Analysis in Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Microscopy.
8: Dual-Focus Fluorescence Correlation Spectroscopy.
9: Pulsed Interleaved Excitation.
10: Image Correlation Spectroscopy Mapping Correlations in Space, Time, and Reciprocal Space.
Author Index.
Subject Index.