eBook Laboratory Methods in Enzymology: RNA, 1st Edition

  • Published By:
  • ISBN-10: 0124200516
  • ISBN-13: 9780124200517
  • DDC: 572.88
  • Grade Level Range: College Freshman - College Senior
  • 472 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013
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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
Other Frontmatter.
Methods in Enzymology.
1: RNA Protocols.
2: Computational Prediction of RNA Secondary Structure.
3: Reverse-Transcription PCR (RT-PCR).
4: Northern Blotting.
5: Explanatory Chapter: Nuclease Protection Assays.
6: In Vitro Transcription.
7: In Vitro Transcription from Plasmid or Pcr-Amplified DNA.
8: Measurement of RNA Synthesis and Decay Rates.
9: Measurement of in Vivo RNA Synthesis Rates.
10: Method for Measuring Mrna Decay Rate in Saccharomyces Cerevisiae.
11: Polysome Analysis.
12: Analysis of Polysomes from Bacteria.
13: Polysome Profile Analysis – Yeast.
14: Polysome Analysis of Mammalian Cells.
15: Polysome Analysis for Determining Mrna and Ribosome Association in Saccharomyces Cerevisiae.
16: Reverse Transcription.
17: Oligo(Dt)-Primed RT-PCR Isolation of Polyadenylated RNA Degradation Intermediates.
18: Circularized RT-PCR (CRT-PCR): Analysis of the 5′ Ends, 3′ Ends, and Poly(A) Tails of RNA.
19: RNA Labeling.
20: RNA Radiolabeling.
21: Fluorescently Labeling Synthetic Rnas.
22: RNA Purification.
23: Analysis of RNA by Analytical Polyacrylamide Gel Electrophoresis.
24: RNA Purification by Preparative Polyacrylamide Gel Electrophoresis.
25: Explanatory Chapter: Nucleic Acid Concentration Determination.
26: RNA Purification – Precipitation Methods.
27: RNA Sequencing.
28: Reverse Transcriptase Dideoxy Sequencing of RNA.
29: RNA Structure Experimental Analysis.
30: RNA Structure Experimental Analysis – Chemical Modification.
31: Structural Analysis of RNA Backbone Using In-Line Probing.
Author Index.
Subject Index.