eBook Laboratory Methods in Enzymology: Protein Part A, 1st Edition

  • Published By:
  • ISBN-10: 0124200974
  • ISBN-13: 9780124200975
  • DDC: 572.6
  • Grade Level Range: College Freshman - College Senior
  • 200 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013
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The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
Other Frontmatter.
1: Protein Protocols.
2: Practical Steady-State Enzyme Kinetics.
3: Quantification of Protein Concentration Using UV Absorbance and Coomassie Dyes.
4: Preparation of Protein Samples for Mass Spectrometry and N-Terminal Sequencing.
5: Protein Protocols/Cell Lysis.
6: Lysis of Mammalian and Sf9 Cells.
7: Protein Protocols/Measurement of Protein Synthesis and Decay Rates.
8: In Vivo [35 S]-Methionine Incorporation.
9: Pulse-Chase Analysis to Measure Protein Degradation.
10: Protein Protocols/Methods for Protein Derivitization.
11: Labeling of a Protein with Fluorophores Using Maleimide Derivitization.
12: Labeling a Protein with Fluorophores Using NHS Ester Derivitization.
13: Protein Derivitization-Expressed Protein Ligation.
14: Protein Biotinylation.
15: Protein Protocols/Protein Expression.
16: Small-Scale Expression of Proteins in E. coli.
17: Protein Expression-Yeast.
18: Recombinant Protein Expression in Baculovirus-Infected Insect Cells.
19: Single Cell Cloning of a Stable Mammalian Cell Line.
Author Index.
Subject Index.