eBook Hydrogen Peroxide and Cell Signaling, Part A, 1st Edition

  • Volume 1
  • Published By:
  • ISBN-10: 0124055427
  • ISBN-13: 9780124055421
  • DDC: 571.6
  • Grade Level Range: College Freshman - College Senior
  • 328 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013
  • Price:  Sign in for price



This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
Methods in Enzymology.
1: Hydrogen Peroxide Detection Methods.
2: Photooxidation of Amplex Red to Resorufin: Implications of Exposing the Amplex Red Assay to Light.
3: Boronate-Based Fluorescent Probes: Imaging Hydrogen Peroxide in Living Systems.
4: Visualization of Intracellular Hydrogen Peroxide with HyPer, a Genetically Encoded Fluorescent Probe.
5: In Vivo Imaging of H2O2 Production in Drosophila.
6: Single Fluorescent Probe Distinguishes Hydrogen Peroxide and Nitric Oxide in Cell Imaging.
7: Electrochemical Biosensors for On-Chip Detection of Oxidative Stress from Cells.
8: Electrochemical Detection of H2O2 Formation in Isolated Mitochondria.
9: Detection of H2O2 by Fluorescence Correlation Spectroscopy.
10: Real-Time Monitoring of Reactive Oxygen and Nitrogen Species in a Multiwell Plate Using the Diagnostic Marker Products of Specific Probes.
11: Temporal and Spatial H2O2 Distribution.
12: H2O2 Delivery to Cells: Steady-State Versus Bolus Addition.
13: Imaging H2O2 Microdomains in Receptor Tyrosine Kinases Signaling.
14: Cellular Sources of H2O2.
15: The Determination and Analysis of Site-Specific Rates of Mitochondrial Reactive Oxygen Species Production.
16: A Microfluidic Systems Biology Approach for Live Single-Cell Mitochondrial ROS Imaging.
17: Detection of Oxidative Damage in Response to Protein Misfolding in the Endoplasmic Reticulum.
Author Index.
Subject Index.