eBook Laboratory Methods in Enzymology: DNA, 1st Edition

  • Published By:
  • ISBN-10: 0124199542
  • ISBN-13: 9780124199545
  • DDC: 572.86
  • Grade Level Range: College Freshman - College Senior
  • 408 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013
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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
Other Frontmatter.
Methods in Enzymology.
1: Explanatory Chapter: PCR Primer Design.
2: Explanatory Chapter: How Plasmid Preparation Kits Work.
3: Explanatory Chapter: Introducing Exogenous DNA into Cells.
4: Agarose Gel Electrophoresis.
5: Analysis of DNA by Southern Blotting.
6: Purification of DNA Oligos by Denaturing Polyacrylamide Gel Electrophoresis (PAGE).
7: Molecular Cloning.
8: Rapid Creation of Stable Mammalian Cell Lines for Regulated Expression of Proteins Using the Gateway® Recombination Cloning Technology and Flp-In T-REx® Lines.
9: Restrictionless Cloning.
10: Isolation of Plasmid DNA from Bacteria.
11: Preparation of Genomic DNA from Bacteria.
12: Preparation of Genomic DNA from Saccharomyces Cerevisiae.
13: Isolation of Genomic DNA from Mammalian Cells.
14: Sanger Dideoxy Sequencing of DNA.
15: Preparation of Fragment Libraries for Next-Generation Sequencing on the Applied Biosystems SOLiD Platform.
16: Explanatory Chapter: Next Generation Sequencing.
17: Generating Mammalian Stable Cell Lines by Electroporation.
18: Transient Mammalian Cell Transfection with Polyethylenimine (PEI).
19: Site-Directed Mutagenesis.
20: PCR-Based Random Mutagenesis.
21: Megaprimer Method for Mutagenesis of DNA.
22: Explanatory Chapter.
23: Explanatory Chapter: Quantitative PCR.
24: General PCR.
25: Colony PCR.
26: Chemical Transformation of Yeast.
27: Transformation of E. coli Via Electroporation.
28: Transformation of Chemically Competent E. coli.
Author Index.
Subject Index.