eBook Endosome Signalling Part A, 1st Edition

  • Volume 1
  • Published By:
  • ISBN-10: 0123984823
  • ISBN-13: 9780123984821
  • DDC: 571.655
  • Grade Level Range: College Freshman - College Senior
  • 412 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013
  • Price:  Sign in for price



This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of two volumes on endosome signaling and includes chapters on such topics as measurement of entry into the endosomal compartment by multi-parametric image analysis, assessment of peptide internalization and endosomal signaling, and VEGF-A in endosomal signaling.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
1: Compartments.
2: Monitoring Phosphatidylinositol 3-Phosphate in Multivesicular Endosome Biogenesis.
3: Methods to Discriminate the Distribution of Acidic Glycohydrolases between the Endosomal–Lysosomal Systems and the Plasma Membrane.
4: Visualizing of Signaling Proteins on Endosomes Utilizing Knockdown and Reconstitution Approach.
5: Virus-Induced Signaling Influences Endosome Trafficking, Virus Entry, and Replication.
6: Methods to Evaluate Zinc Transport into and out of the Secretory and Endosomal–Lysosomal Compartments in dt40 Cells.
7: Interactions between Endosomal Maturation and Autophagy: Analysis of ESCRT Machinery during Caenorhabditis Elegans Development.
8: Assessment of Cation Trapping by Cellular Acidic Compartments.
9: Signaling Initiated by the Secretory Compartment.
10: Image-Based and Biochemical Assays to Investigate Endosomal Protein Sorting.
11: Transport and Transfer.
12: Cytokines, Polarity Proteins, and Endosomal Protein Trafficking and Signaling—the Sertoli Cell Blood–Testis Barrier System in Vitro as a Study Model.
13: Methods of Analysis of the Membrane Trafficking Pathway from Recycling Endosomes to Lysosomes.
14: Measurement of Intercellular Transfer to Signaling Endosomes.
15: Liposome-Based Assays to Study Membrane-Associated Protein Networks.
16: Mouse Models of PI(3,5)P2 Deficiency with Impaired Lysosome Function.
17: Monitoring Endosomal Trafficking of the G Protein-Coupled Receptor Somatostatin Receptor 3.
18: Proteins.
19: Genetic Circuitry Modulating Notch Signals through Endosomal Trafficking.
20: Monitoring Notch/Delta Endosomal Trafficking and Signaling in Drosophila.
21: Toll-Interacting Protein Pathway: Degradation of an Ubiquitin-Binding Protein.
22: Measuring Interactions of FERM Domain-Containing Sorting Nexin Proteins with Endosomal Lipids and Cargo Molecules.
Author Index.
Subject Index.
Other Backmatter.