eBook G Protein Coupled Receptors, 1st Edition

  • Volume 521
  • Published By:
  • ISBN-10: 0123918731
  • ISBN-13: 9780123918734
  • DDC: 572.696
  • Grade Level Range: College Freshman - College Senior
  • 480 Pages | eBook
  • Original Copyright 2013 | Published/Released June 2014
  • This publication's content originally published in print form: 2013
  • Price:  Sign in for price



This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high throughput assays for identification of pharmacoperone drugs.

Table of Contents

Front Cover.
Half Title Page.
Other Frontmatter.
Title Page.
Copyright Page.
Methods in Enzymology.
1: GPCR Trafficking.
2: Therapeutic Rescue of Misfolded/Mistrafficked Mutants: Automation-Friendly High-Throughput Assays for Identification of Pharmacoperone Drugs of GPCRs.
3: Trafficking of the Follitropin Receptor.
4: Single-Molecule Imaging Technique to Study the Dynamic Regulation of GPCR Function at the Plasma Membrane.
5: GPCR Oligomerization and Receptor Trafficking.
6: β-Arrestins and G Protein-Coupled Receptor Trafficking.
7: Tracking Cell Surface Mobility of GPCRs Using α-Bungarotoxin-Linked Fluorophores.
8: Regulatory Mechanism of G Protein-Coupled Receptor Trafficking to the Plasma Membrane: A Role for mRNA Localization.
9: Dissecting Trafficking and Signaling of Atypical Chemokine Receptors.
10: Trafficking Motifs.
11: Systematic and Quantitative Analysis of G Protein-Coupled Receptor Trafficking Motifs.
12: Identification of Endoplasmic Reticulum Export Motifs for G Protein-Coupled Receptors.
13: Amino Acid Residues of G-Protein-Coupled Receptors Critical for Endoplasmic Reticulum Export and Trafficking.
14: GPCR Oligomerization.
15: G-Protein-Coupled Heteromers:Regulation in Disease.
16: Hetero-oligomerization and Specificity Changes of G Protein-Coupled Purinergic Receptors Novel Insight into Diversification of Signal Transduction.
17: Bimolecular Fluorescence Complementation Analysis of G Protein-Coupled Receptor Dimerization in Living Cells.
18: G Protein–Coupled Receptor Heterodimerization in the Brain.
19: Experimental Strategies for Studying G Protein-Coupled Receptor Homo- and Heteromerization with Radioligand Binding and Signal Transduction Methods.
20: Analysis of GPCR Dimerization Using Acceptor Photobleaching Resonance Energy Transfer Techniques.
21: Techniques for the Discovery of GPCR-Associated Protein Complexes.
22: Expression, Purification, and Analysis of G-Protein-Coupled Receptor Kinases.
23: Modern Methods to Investigate the Oligomerization of Glycoprotein Hormone Receptors (TSHR, LHR, FSHR).
Color Plate.
Author Index.
Subject Index.