Higher Education

Microbiology Laboratory Manual, 1st Edition

  • Russell Bey University of Minnesota
  • ISBN-10: 0534375642  |  ISBN-13: 9780534375645
  • 208 Pages
  • © 2001 | Published
  • College Bookstore Wholesale Price = $158.25



This manual contains a carefully selected set of lab exercises covering both classic and state-of-the-art experiments, with an emphasis on the development of critical-thinking skills and an understanding of the scientific method. It offers the opportunity to implement innovative, engaging, and effective labs, which have been class-tested for years.

Table of Contents

General Laboratory Directions.
Exercise 1: Ubiquity of Microorganisms.
Exercise 2: Effectiveness of Hand Washing.
Exercise 3: Microscopy.
Exercise 4: Eukaryotes - The Larger Microorganisms.
Part A: Fungi and Terrestrial Protozoa/ Part B: Algae/ Part C: Aquatic Protozoa.
Exercise 5: Handling of Microorganisms.
Part A: Slide Preparations/ Part B: Introduction to Staining.
Exercise 6: Aseptic Transfer.
Exercise 7: Pure Culture Isolation of Bacteria.
Part A: Enrichment Culture/ Part B: The Streak-Plate Isolation.
Exercise 8: Gross Anatomy of Bacteria.
Part A: Flagella/ Part B: Endospores.
Exercise 9: The Culture Medium.
Part A: Sources of Carbon, Nitrogen and Energy/ Part B: Selective Media.
Exercise 10: Growth Features of Bacteria and Fungi.
Part A: Features of the Fungi/ Part B: Cultured Features of Bacteria.
Exercise 11: Effects of Environment on Microorganisms.
Part A: Oxygen/ Part B: Temperature.
Exercise 12: Extracellular Hydrolytic Enzymes.
Part A: Hydrolysis of Starch/ Part B: Casein Hydrolysis/ Part C: Hydrolysis of Gelatin.
Exercise 13: Influence of High Temperatures on Bacteria.
Exercise 14: Enumeration of Bacteria and Growth Curve.
Exercise 15: Chemotactic and Phototropic Response to Environment.
Part A: Chemotaxis in Bacteria/ Part B: Phototropism in Pilobolus.
Exercise 16: Bacterial Transformation.
Exercise 17: Drug Resistance Transfer Between Bacteria.
Exercise 18: Viruses of Bacteria.
Exercise 19: Destruction and Inhibition of Microorganisms.
Part A: Antiseptics and Disinfectants/ Part B: Action of Antibiotics.
Exercise 20: The Nitrogen-Fixing Bacteria.
Exercise 21: Microbiological Examination of Milk.
Part A: Agar Plate Count/ Part B: Coliform Count.
Exercise 22: Microbiological Analysis of Food Products.
Exercise 23: Sanitary Examination of Water.
Part A: Examination of Water for Coliform Bacteria/ Part B: The �IMViC� Test/ Part C: Demonstration of Membrane Filtration.
Exercise 24: Action of Antibiotics.
Part A: Antibiotic Production as Determined Qualitatively by the Cross-Streak Plate Method/ Part B: Paper Disc Method for Testing Antibiotic Sensitivity.
Exercise 25: Serological Reactions.
Exercise 26: The Pathogenic Cocci.
Exercise 27: The Gram-Positive Pathogenic Rods.
Part A: Gram-Positive Endospore-Forming Rods/ Part B: Gram-Positive Nonsporing Rods.
Exercise 28: The Fermentative Gram-Negative Rods.
Exercise 29: Identification of Unknowns.
Exercise 30: Group Project.