EXPERIMENTS IN BIOCHEMISTRY: A HANDS-ON APPROACH, Second Edition features a variety of hands-on, classroom tested experiments that are proven to work and can be completed in a normal lab period. The manual's stand-alone experiments are effective in courses meeting only once a week, giving students a broad overview of the subject matter. A more comprehensive set of experiments is also available and allows students to delve further into each of the topics presented. The Second Edition also features new and revised experiments, including a new experiment that involves cloning the barracuda LDH gene! Students and professors will also find expanded problem sets in this edition. "Tip" boxes, located throughout the text, provide pointers to students on how to perform the experiment at hand, while "Essential Information" boxes highlight pertinent information that will help the student complete the experiment. The second edition continues to include references and further readings at the end of each chapter.
Table of Contents
Introduction to the Text.
Objectives of the Biochemistry Laboratory.
Chapter Format of Hands on Biochemistry.
1. Biochemistry Boot Camp.
2. Acids, Bases, and Buffers.
4. Enzyme Purification.
5. Ion Exchange Chromatography.
6. Affinity Chromatography.
7. Gel filtration Chromatography.
8. Enzyme Kinetics.
10. Western Blots.
11. Restriction Enzymes.
12. Cloning and Expression of Foreign Proteins.
13. Polymerase Chain Reaction.